Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand.

نویسندگان

  • Alan Brockman
  • Sittaporn Singlam
  • Lucy Phiaphun
  • Sornchai Looareesuwan
  • Nicholas J White
  • François Nosten
چکیده

A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/microl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 48 4  شماره 

صفحات  -

تاریخ انتشار 2004